Open AccessCan the grey mould disease of the grape‐vine be controlled by yeast?
Author(s) -
Masih Emmanuel Isaac,
Alie Isabelle,
Paul Bernard
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09236.x
Subject(s) - botrytis cinerea , yeast , biology , vine , inoculation , botrytis , anomala , pathogen , botany , microbiology and biotechnology , horticulture , genetics
Botrytis cinerea has been found to be highly pathogenic to ‘Chardonnay’ and ‘Pinot noir’ cultivars of the grape‐vine producing the characteristic grey mould symptoms within 7 days of inoculation to the vitro‐plants. The yeast Pichia anomala (strain FY‐102), isolated from apple skin, was found to be antagonistic to B. cinerea as it completely inhibited the appearance of the grey mould symptoms when grown together. The yeast was responsible for morphological changes such as coagulation and leakage of the cytoplasm of B. cinerea . The pathogen, when applied together with P. anomala , failed to bring about the grey mould symptoms on the grape‐vine, suggesting that the yeast could control the expression of this disease. An account of the interaction between B. cinerea and P. anomala , as well as the sequences of the complete ITS region of the ribosomal DNA of the yeast are described here.