Open AccessA variation of the amplified‐fragment length polymorphism (AFLP) technique using three restriction endonucleases, and assessment of the enzyme combination Bgl II– Mfe I for AFLP analysis of Salmonella enterica subsp. enterica isolates
Author(s) -
Lindstedt BjørnArne,
Heir Even,
Vardund Traute,
Kapperud Georg
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09200.x
Subject(s) - amplified fragment length polymorphism , bglii , restriction enzyme , biology , genotyping , salmonella enterica , genetics , dna profiling , ecori , salmonella , restriction fragment length polymorphism , terminal restriction fragment length polymorphism , restriction fragment , microbiology and biotechnology , genotype , dna , gene , bacteria , genetic diversity , population , sociology , demography
We have performed amplified‐fragment length polymorphism (AFLP) fingerprinting on a collection of Salmonella enterica subsp. enterica serovar typhimurium strains with a restriction endonuclease combination ( Bgl II and Mfe I) that has previously been used successfully for typing Campylobacter jejuni isolates with high resolution. Additionally, a variation of the AFLP assay in which two rare cutting restriction enzymes ( Xba I and Bsr GI) in combination with the frequent cutter ( Hin P1I) was examined. The Bgl II and Mfe I enzyme combination offered low resolution for genotyping Salmonella typhimurium isolates and is not recommended for this common serovar. The three‐enzyme combination gave a higher discrimination, and is thus a new alternate way of performing AFLP fingerprinting of S. typhimurium .