Open AccessIdentification of type 4 fimbriae in Actinobacillus pleuropneumoniae
Author(s) -
Zhang Yamei,
Tennent Jan M,
Ingham Aaron,
Beddome Gary,
Prideaux Christopher,
Michalski Wojtek P
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09199.x
Subject(s) - fimbria , actinobacillus pleuropneumoniae , polyclonal antibodies , microbiology and biotechnology , protein subunit , biology , pilus , peptide sequence , escherichia coli , antibody , biochemistry , serotype , gene , genetics
Type 4 fimbriae have been identified on the cell surface of Actinobacillus pleuropneumoniae by electron microscopy and N‐terminal sequencing analysis. A. pleuropneumoniae type 4 fimbrial subunit protein, purified from cell cultures and from outer membrane preparations, reacted with polyclonal antibody raised against type 4 fimbriae of Moraxella bovis on Western blots. N‐terminal sequence analysis of the purified 17 kDa type 4 fimbrial subunit protein, named ApfA, revealed the first 12 amino acids to be identical to those of other type 4 fimbrial subunit proteins.