Open AccessAntibody engineering and its applications in tumor targeting and intracellular immunization
Author(s) -
Chames Patrick,
Baty Daniel
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09197.x
Subject(s) - computational biology , recombinant dna , antibody , selection (genetic algorithm) , intracellular , immunization , phage display , escherichia coli , biology , microbiology and biotechnology , computer science , immunology , genetics , gene , artificial intelligence
During the last decade, recombinant antibody engineering has emerged as one of the most promising approaches for the design, selection and production of molecules for basic research, medicine and the pharmaceutical industry. This MiniReview describes the major findings that have led to the development of this powerful technique, with an emphasis on the use of Escherichia coli and filamentous phage as a tool allowing powerful selection procedures from large libraries as well as the use of intracellular expression of antibody fragments as a new class of neutralizing molecules with a potential use in therapy. The future of these rapidly evolving technologies is discussed.