Open AccessIsolation and characterization of an Achromobacter xylosoxidans strain B3 and other bacteria capable to degrade the synthetic chelating agent iminodisuccinate
Author(s) -
Reinecke Frank,
Groth Torsten,
Heise KlausPeter,
Joentgen Winfried,
Müller Nikolaus,
Steinbüchel Alexander
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09166.x
Subject(s) - achromobacter xylosoxidans , achromobacter , axenic , bacteria , 16s ribosomal rna , strain (injury) , isolation (microbiology) , microbiology and biotechnology , chelation , enrichment culture , chemistry , biology , pseudomonas , organic chemistry , genetics , anatomy
Three bacterial strains were isolated, which used the synthetic chelating agent iminodisuccinate (IDS) as sole carbon source for growth in mineral salts media (MSM). Taxonomic analysis and 16S rDNA sequence analysis identified one of these isolates (B3), which was isolated from sewage sludge, as a strain of Achromobacter xylosoxidans subsp. xylosoxidans . It exhibited a doubling time of approximately 3 h in liquid MSM supplemented with IDS and grew even in the presence of 1.0% (w/v) IDS. Since photometric and high performance liquid chromatography analysis showed that IDS, which came onto the market only recently as an alternative for ethylenediaminetetraacetate, was completely degraded by axenic cultures of bacteria; it will probably be readily degraded in the environment.