Open AccessResponse to nitrogen starvation in Corynebacterium glutamicum
Author(s) -
Schmid Roland,
Uhlemann EvaMaria,
Nolden Lars,
Wersch Gregor,
Hecker Rolf,
Hermann Thomas,
Marx Achim,
Burkovski Andreas
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09141.x
Subject(s) - corynebacterium glutamicum , biochemistry , enzyme , nitrogen , bacteria , biology , metabolism , gene , gel electrophoresis , starvation , corynebacterium , chemistry , genetics , organic chemistry , endocrinology
Proteins strongly synthesized in Corynebacterium glutamicum during nitrogen restriction were examined by two‐dimensional gel electrophoresis and microsequencing. Two main groups of enzymes were identified beside miscellaneous proteins, enzymes involved (i) in protein synthesis, and (ii) in carbon metabolism. Biochemical measurements revealed an increase of oxygen consumption during nitrogen starvation, indicating an enhanced energy demand of the cells. By Northern hybridizations, an increased transcription for the gap and fda genes upon nitrogen deprivation was shown.