Open AccessRepression of Acinetobacter vanillate demethylase synthesis by VanR, a member of the GntR family of transcriptional regulators
Author(s) -
Morawski Birgit,
Segura Ana,
Ornston L.Nicholas
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09138.x
Subject(s) - repressor , demethylase , gene , open reading frame , chemistry , strain (injury) , biology , psychological repression , genetics , microbiology and biotechnology , transcription factor , gene expression , peptide sequence , epigenetics , anatomy
Vanillate is converted to protocatechuate by the action of vanillate demethylase encoded by vanAB . Convergent upon and overlapping Acinetobacter vanB is an open reading frame encoding a member of the gntR repressor family and designated vanR . This gene organization differs from that found in a Pseudomonas isolate. An Acinetobacter strain with a knockout mutation in vanR constitutively converted vanillate to protocatechuate. Reverse transcriptase‐polymerase chain reaction was used to demonstrate that control of vanAB was exerted at the level of transcription.