Open AccessOutbreak of Klebsiella pneumoniae producing transferable AmpC‐type β‐lactamase (ACC‐1) originating from Hafnia alvei
Author(s) -
Nadjar David,
Rouveau Martine,
Verdet Charlotte,
Donay JeanLuc,
Herrmann JeanLouis,
Lagrange Philippe H.,
Philippon Alain,
Arlet Guillaume
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09133.x
Subject(s) - klebsiella pneumoniae , microbiology and biotechnology , hafnia , outbreak , enterobacteriaceae , klebsiella , biology , virology , escherichia coli , chemistry , gene , genetics , cubic zirconia , ceramic , organic chemistry
Fifty‐two strains of Klebsiella pneumoniae producing an AmpC‐type plasmid‐mediated β‐lactamase were isolated from 13 patients in the same intensive care unit between March 1998 and February 1999. These strains were resistant to ceftazidime, cefotaxime and ceftriaxone, but susceptible to cefoxitin, cefepime and aztreonam. Plasmid content and genomic DNA restriction pattern analysis suggested dissemination of a single clone. Two β‐lactamases were identified, TEM‐1 and ACC‐1. We used internal bla ACC‐1 primers, to sequence PCR products obtained from two unrelated strains of Hafnia alvei . Our results show that the ACC‐1 β‐lactamase was derived from the chromosome‐encoded AmpC‐type enzyme of H. alvei .