Open AccessDetection of cysteine‐proteinases in Leishmania amazonensis promastigotes using a cross‐reactive antiserum
Author(s) -
Alves Carlos Roberto,
CôrteReal Suzana,
DeFreitas Rosa Marcelo,
GiovanniDeSimone Salvatore
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09115.x
Subject(s) - antiserum , cysteine , leishmania , papain , biology , biochemistry , cysteine proteinase inhibitors , microbiology and biotechnology , leishmania mexicana , kinetoplastida , cystatin , flagellum , proteolysis , enzyme , chemistry , parasite hosting , cystatin c , antibody , immunology , apoptosis , protozoal disease , programmed cell death , renal function , world wide web , computer science , malaria , gene , caspase
Rabbit serum against the cysteine‐proteinases papain has been employed for the cellular localization of cysteine‐proteinases of in Leishmania amazonensis promastigotes. By immunocytochemistry, immune complexes were found in the plasma membrane and in the flagella pocket of the parasite. The antiserum immunoprecipitated major iodinated proteins with molecular masses of 66, 45, 28 and 24 kDa and a wide partitioning of the Triton X‐114 detergent phase. The presence of cysteine‐proteinase at the cell surface membrane was also suggested by the detection of proteolytic activity in living cells (19.0 μg azocasein min −1 10 −7 promastigotes (1.0 S.D.)).