Open AccessNegative regulation of the bolA1p of Escherichia coli K‐12 by the transcription factor OmpR for osmolarity response genes
Author(s) -
Yamamoto Kaneyoshi,
Nagura Rie,
Tanabe Hiroyuki,
Fujita Nobuyuki,
Ishihama Akira,
Utsumi Ryutaro
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09114.x
Subject(s) - transcription factor , transcription (linguistics) , biology , gene , escherichia coli , promoter , microbiology and biotechnology , regulator , genetics , gene expression , linguistics , philosophy
To test whether OmpR is involved in regulation of the bolA1p , we investigated possible effects of ompR mutation on transcription from bolA1p . In vivo, bolA1p was found to be repressed by OmpR. Furthermore in vitro, the phospho‐OmpR was found to bind to the OmpR binding region of bolA1p and repress the transcription by Eσ S or Eσ D . These results suggest that the phosphorylated form of OmpR is a negative regulator for the transcription of the bolA1p promoter.