Open AccessOrganophosphonate metabolism by a moderately halophilic bacterial isolate
Author(s) -
Hayes Velma E.A,
Ternan Nigel G,
McMullan Geoffrey
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09099.x
Subject(s) - halophile , pseudomonas , metabolism , biochemistry , biology , strain (injury) , microbiology and biotechnology , pseudomonadales , bacteria , chemistry , anatomy , genetics
A Gram‐negative halophile isolated from soil beneath a road gritting salt pile grew optimally at 10% (w/v) NaCl and was shown most likely to be Chromohalobacter marismortui or Pseudomonas beijerinckii on the basis of 16S rRNA analysis. The strain utilised phosphonoacetate, 2‐aminoethyl‐, 3‐aminopropyl‐, 4‐aminobutyl‐, methyl‐ and ethyl‐phosphonates as phosphorus sources for growth. Differences were observed in the growth rate on different phosphonates and the range of phosphonates utilised at elevated NaCl concentrations, possibly as a result of differentially‐induced transport mechanisms. An assay of cell‐free extracts of 2‐aminoethylphosphonate (2AEP) grown cells showed no detectable 2AEP:pyruvate aminotransferase or phosphonoacetaldehyde hydrolase activity.