Open AccessMembrane proteins PmpG and PmpH are major constituents of Chlamydia trachomatis L2 outer membrane complex
Author(s) -
Mygind Per Holse,
Christiansen Gunna,
Roepstorff Peter,
Birkelund Svend
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09098.x
Subject(s) - chlamydia trachomatis , chlamydiaceae , bacterial outer membrane , chlamydiales , membrane , membrane protein , chemistry , chlamydia , microbiology and biotechnology , biology , virology , biochemistry , immunology , escherichia coli , gene
The outer membrane complex of Chlamydia is involved in the initial adherence and ingestion of Chlamydia by the host cell. In order to identify novel proteins in the outer membrane of Chlamydia trachomatis L2, proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis. By silver staining of the protein profile, a major protein doublet of 100–110 kDa was detected. In‐gel tryptic digestion and matrix‐assisted laser desorption/ionization mass spectrometry identified these proteins as the putative outer membrane proteins PmpG and PmpH.