Open AccessTransposon targeting determined by resolvase
Author(s) -
KamaliMoghaddam Masood,
Sundström Lars
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09081.x
Subject(s) - tn3 transposon , transposable element , recombinase , transposition (logic) , site specific recombination , genetics , plasmid , biology , gene , transposase , recombination , serine , mutant , computer science , phosphorylation , artificial intelligence
The Mu‐related transposon Tn 5090 , also called Tn 402 , was observed to be highly selective for targets clustered in or close to recombination sites of serine‐type recombinases in plasmids R388 and RP1. Transposition to the par area of RP1 responded strongly to a deletion in the gene of resolvase ParA. A search in sequence databanks revealed further insertions of Tn 5090 /Tn 402 close to different genes of resolvases. These results imply that the target selection of Tn 5090 depends on a property that is shared among several serine recombinases.