Open AccessIsolation and characterization of alkane‐utilizing Nocardioides sp. strain CF8
Author(s) -
Hamamura Natsuko,
Arp Daniel J
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09076.x
Subject(s) - monooxygenase , strain (injury) , butane , alkane , thermophile , stereochemistry , chemistry , 16s ribosomal rna , organic chemistry , microbiology and biotechnology , biology , biochemistry , hydrocarbon , enzyme , gene , catalysis , cytochrome p450 , anatomy
A butane‐utilizing bacterial strain CF8 was isolated and identified as a member of the genus Nocardioides from chemotaxonomic and 16S rDNA sequence analysis. Strain CF8 grew on alkanes ranging from C 2 to C 16 in addition to butane and various other substrates including primary alcohols, carboxylic acids, and phenol. Butane degradation by strain CF8 was inactivated by light, a specific inactivator of copper‐containing monooxygenases. The unique thermal aggregation phenomenon of acetylene‐binding polypeptides was also observed for strain CF8. These results suggest that butane monooxygenase in strain CF8 is a third example of the copper‐containing monooxygenases previously described in ammonia oxidizers and methanotrophs.