Open AccessEscherichia coli O157:H7 induces attaching‐effacing lesions in large intestinal mucosal explants from adult cattle
Author(s) -
Baehler Angela A,
Moxley Rodney A
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09068.x
Subject(s) - weanling , escherichia coli , microbiology and biotechnology , biology , explant culture , epithelium , inoculation , cecum , bacteria , intestinal epithelium , intestinal mucosa , pathology , immunology , in vitro , medicine , endocrinology , ecology , biochemistry , genetics , gene
Attaching‐effacing (A/E) lesions following natural and experimental infection with Escherichia coli O157:H7 have been seen in neonatal and 3–4‐month‐old weanling but not older cattle. To test the hypothesis that the adult bovine large intestinal epithelium is resistant to the development of A/E lesions, colonic and rectal mucosal tissue explants from 18‐month‐old steers were inoculated with E. coli O157:H7 and examined. Epithelial cells of inoculated explants developed A/E lesions at the bacterial attachment sites, providing evidence that the large intestinal mucosal epithelium may be a site of infection that contributes to carriage of E. coli O157:H7 in adult cattle.