Open AccessA Brevibacterium lactofermentum 16S rRNA gene used as target site for homologous recombination
Author(s) -
Amador Eladio,
Martín Juan Francisco,
Castro José María
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09062.x
Subject(s) - homologous recombination , operon , genetics , biology , gene , plasmid , brevibacterium , horizontal gene transfer , ribosomal rna , dna , 16s ribosomal rna , genome , bacteria , escherichia coli , microorganism
Genes for rRNA are highly conserved and present in multiple copies in most prokaryotic organisms increasing the number of theoretical sites for homologous recombination. They might be targets for integration events between unrelated microorganisms providing that an efficient genetic transfer is present. We have used a plasmid containing a portion of the 16S rRNA gene from the rrn D operon of Brevibacterium lactofermentum to transform the same strain resulting in non‐essential inactivation of various rrn operons. Integration of the transforming DNA occurs in all cases. The system may be used to test possible gene transfer at least among closely related strains and is of great interest for integration of foreign DNA and for mapping.