Open AccessAnalysis of Leptospira isolates from mainland Portugal and the Azores islands
Author(s) -
CollaresPereira M,
Korver H,
Cao Thi B.V,
SantosReis M,
Bellenger E,
Baranton G,
Terpstra W.J
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09059.x
Subject(s) - leptospira , leptospira interrogans , pulsed field gel electrophoresis , serotype , biology , microbiology and biotechnology , polymerase chain reaction , serology , agarose gel electrophoresis , genetics , genotype , gene , antibody
From 228 recent Leptospira isolates from mainland Portugal and Azorean wild mammals, 149 were characterized at the serovar level by monoclonal antibodies (MAbs), a quick serological method in epidemiological studies. In order to compare this antigenic information with that from new genetic techniques, a sample of isolates was analyzed through pulsed‐field agarose gel electrophoresis (PFGE) ( n =71 ), mapped restriction site polymorphisms (MRSPs) in PCR‐amplified rRNA genes ( n =45 , including 13 saprophytes) and arbitrarily primed polymerase chain reaction (AP‐PCR) fingerprinting ( n =32 ). MRSP and AP‐PCR lead to species identification of the studied 32 pathogenic isolates: Leptospira interrogans ( n =3 ), Leptospira kirschneri ( n =8 ) and Leptospira borgpetersenii ( n =21 ). MAbs and PFGE characterized pathogenic isolates at the serovar level and resulted mainly in agreement (64%) although many discrepancies (35%) were observed.