Open AccessRibotyping and rapid identification of Staphylococcus xylosus by 16–23S spacer amplification
Author(s) -
Villard Laurence,
Kodjo Angeli,
Borges Evelyne,
Maurin Françoise,
Richard Yves
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09044.x
Subject(s) - ribotyping , staphylococcus xylosus , microbiology and biotechnology , staphylococcus , biology , staphylococcus aureus , micrococcaceae , ribosomal dna , genetics , polymerase chain reaction , bacteria , phylogenetics , gene
Ninety‐five strains of Staphylococcus xylosus isolated from goat milk, French sausage or mice were analyzed together with 35 Staphylococcus type strains by 16–23S spacer amplification and ribotyping. The results obtained by PCR amplification of the 16–23S spacer region permitted the distinction of each type strain and additionally generated a DNA banding pattern characteristic for 93 of the 95 Staphylococcus xylosus strains. Ribotyping proved to be an efficient epidemiological tool for Staphylococcus xylosus species as it clustered the 95 strains into 23 distinct types.