Open AccessPlasmid curing effect of trovafloxacin
Author(s) -
Brandi Letizia,
Falconi Maurizio,
Ripa Sandro
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09030.x
Subject(s) - trovafloxacin , plasmid , escherichia coli , dna supercoil , microbiology and biotechnology , dna , minimum inhibitory concentration , chemostat , bacteria , biology , chemistry , dna replication , antibiotics , antibacterial agent , genetics , gene
The effect of sub‐inhibitory concentrations of trovafloxacin, a recently developed fluoroquinolone molecule, on the capability of Escherichia coli cells to maintain three different types of plasmids has been investigated by a number of approaches, including the quantification of the loss of plasmid‐borne functions and of plasmid DNA by quantitative PCR. The results obtained demonstrate that at concentrations ranging from the MIC to 1/8 of the MIC, trovafloxacin induces a clear, albeit incomplete, ‘episome‐curing’ effect which was observed with plasmids differing in copy number, size and nature of the replication origin of the episome. This effect was most likely not due to an alteration of DNA supercoiling.