Open AccessEvidence for the transport of zinc(II) ions via the Pit inorganic phosphate transport system in Escherichia coli
Author(s) -
Beard Steven J.,
Hashim Rohani,
Wu Guanghui,
Binet Marie R.B.,
Hughes Martin N.,
Poole Robert K.
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09019.x
Subject(s) - zinc , escherichia coli , mutant , phosphate , transposon mutagenesis , chemistry , transposable element , biochemistry , mutagenesis , inorganic phosphate , enterobacteriaceae , site directed mutagenesis , biology , microbiology and biotechnology , gene , organic chemistry
A locus involved in zinc(II) uptake in Escherichia coli K‐12 was identified through the generation of a zinc(II)‐resistant mutant by transposon (Tn 10 dCam) mutagenesis. The mutation was located within the pitA gene, which encodes the low‐affinity inorganic phosphate transport system (Pit). The pitA mutant accumulated reduced amounts of zinc(II) when exposed to 0.5–2.0 mM ZnSO 4 during growth in Luria–Bertani medium.