Open AccessIdentification of iron‐regulated genes of Helicobacter pylori by a modified Fur titration assay (FURTA‐Hp)
Author(s) -
Fassbinder Frank,
Vliet Arnoud H.M.,
Gimmel Verena,
Kusters Johannes G.,
Kist Manfred,
Bereswill Stefan
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09018.x
Subject(s) - escherichia coli , helicobacter pylori , biology , gene , bacteria , microbiology and biotechnology , biochemistry , genetics
The Escherichia coli ‐based Fur titration assay (FURTA), although a powerful tool for identification of genes regulated by the ferric uptake regulator (Fur), was unsuccessful for the gastric pathogen Helicobacter pylori . The FURTA was modified by construction of an E. coli indicator strain producing H. pylori Fur only. The promoter regions of the ferric citrate receptor homolog fecA2 and the riboflavin synthesis gene ribBA were both positive in the modified FURTA, but negative in the original FURTA. Transcription of fecA2 and ribBA was demonstrated to be iron‐repressed in H. pylori . This type of modification should allow FURTA analysis for bacteria with Fur binding sequences poorly recognized by E. coli Fur.