Open AccessProtein interactions of SGP, an essential Streptococcus mutans GTPase, revealed by biochemical and yeast two‐hybrid system analyses
Author(s) -
Baev Didi,
Ohk SeungHo,
Kuramitsu Howard K
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09006.x
Subject(s) - yeast , immunoprecipitation , gtpase , function (biology) , biology , gtp' , biochemistry , two hybrid screening , microbiology and biotechnology , chemistry , gene , enzyme
SGP, a Streptococcus mutans essential GTPase, plays a role in the stress response of the organism. Recently, we proposed that one of the physiological functions of the SGP is the modulation of the GTP/GDP ratio under different growth conditions. In order to further determine the functions of SGP and its possible interactions with other molecules, we carried out immunoprecipitation, SGP binding, and the yeast two‐hybrid system analyses. These approaches suggest that SGP may oligomerize and such interactions could be important for the function of this regulatory protein.