Open AccessIsolation of U(VI) reduction‐deficient mutants of Shewanella putrefaciens
Author(s) -
Wade Roy,
DiChristina Thomas J
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09005.x
Subject(s) - shewanella putrefaciens , isolation (microbiology) , microbiology and biotechnology , mutant , shewanella , chemistry , reduction (mathematics) , biology , bacteria , genetics , biochemistry , gene , mathematics , geometry
A U(VI) reduction‐deficient mutant (Urr) screening technique was developed and combined with chemical mutagenesis procedures to identify a Urr mutant of Shewanella putrefaciens strain 200. The Urr mutant lacked the ability to grow anaerobically on U(VI) and NO 2 − , yet retained the ability to grow anaerobically on eight other compounds as terminal electron acceptor. All 11 members of previously isolated sets of Fe(III) and Mn(IV) reduction‐deficient mutants of S. putrefaciens 200 displayed Urr‐positive phenotypes with the Urr screen and were capable of anaerobic growth on U(VI). This is the first reported isolation of a respiratory mutant that is unable to grow anaerobically on U(VI) as terminal electron acceptor.