Open AccessClonal turnover of enterohemorrhagic Escherichia coli O157:H7 in experimentally infected cattle
Author(s) -
Akiba Masato,
Sameshima Toshiya,
Nakazawa Muneo
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb08994.x
Subject(s) - escherichia coli , biology , inoculation , microbiology and biotechnology , clone (java method) , bacteria , virology , genetics , gene , immunology
A total of 401 enterohemorrhagic Escherichia coli (EHEC) O157:H7 isolates from two experimentally infected calves were analyzed using molecular biological methods. Genetic differences detected by pulsed‐field gel electrophoresis were observed between the inoculated and recovered strains as early as 1 day post inoculation. The loss of the inoculated clone was observed in one calf. Replication and dissemination of the EHEC O157:H7 strains that mutated in cattle may result in the diversification of this organism among cattle populations.