Open AccessMannanase Man26A from Cellulomonas fimi has a mannan‐binding module
Author(s) -
Stoll Dominik,
Boraston Alisdair,
Stålbrand Henrik,
McLean Bradley W.,
Kilburn Douglas G.,
Warren R.Antony J.
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb08969.x
Subject(s) - mannan , chitin , locust bean gum , chemistry , carbohydrate binding module , cellulose , xylan , biochemistry , glycoside hydrolase , cellulase , polysaccharide , enzyme , chitosan , materials science , xanthan gum , rheology , composite material
A modular mannanase (Man26A) from the bacterium Cellulomonas fimi contains a mannan‐binding module (Man26Abm) that binds to soluble but not to insoluble mannans. Man26Abm does not bind to cellulose, chitin or xylan. The K d for binding of Man26Abm to locust bean gum (LBG) is ∼0.2 μM. Man26A is the first mannanase reported to contain a mannan‐binding module.