Open AccessSynthesis of cefminox by cell‐free extracts of Streptomyces clavuligerus
Author(s) -
Kim Jeong Keun,
Kang Heuiil,
Chae Jeong Seok,
Park YoungHoon,
Choi YongJin
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb08914.x
Subject(s) - streptomyces clavuligerus , methionine , biochemistry , chemistry , streptomyces , ascorbic acid , in vitro , biology , amino acid , bacteria , food science , clavulanic acid , antibiotics , genetics , amoxicillin
In vitro synthesis of cefminox by cell‐free extracts of Streptomyces clavuligerus was investigated using α‐ketoglutarate, L ‐ascorbic acid, FeSO 4 , S ‐adenosyl‐ L ‐methionine, and 7α‐demethoxycefminox as the substrates. The formation of cefminox was detected both by a biological assay with Proteus vulgaris GN 76/C‐1 and by high performance liquid chromatography. Although the conversion rate of 7α‐demethoxycefminox to cefminox was observed to be quite low, it still demonstrated the potential for an enzymatic process to replace the chemical steps which are currently in use for the production of cefminox.