Open AccessIdentification of four loci isolated from two Streptococcus thermophilus phage genomes responsible for mediating bacteriophage resistance
Author(s) -
Stanley Elizabeth,
Walsh Linda,
Zwet Anneke,
Fitzgerald Gerald F.,
Sinderen Douwe
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb08907.x
Subject(s) - streptococcus thermophilus , bacteriophage , biology , genome , genetics , southern blot , dna , replication (statistics) , dna replication , microbiology and biotechnology , gene , bacteria , virology , escherichia coli , lactobacillus
Sequence data derived from the Streptococcus thermophilus phages φO1205 and φ7201 indicated that each of these phages contains a distinct DNA region dedicated to replication. Southern blotting experiments showed that phages infecting S. thermophilus may be divided into at least two groups, each containing the presumptive replication functions of either φO1205 (group I) or φ7201 (group II). Specific regions from the putative replication module of each of the two phages were examined for their ability to provide phage resistance.