Open AccessAn aerotaxis transducer gene from Pseudomonas putida
Author(s) -
Nichols Nancy N.,
Harwood Caroline S.
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb08893.x
Subject(s) - pseudomonas putida , mutant , wild type , biology , chemistry , gene , biochemistry
An aerotaxis gene, aer , was cloned from Pseudomonas putida PRS2000. A P. putida aer mutant displayed an altered aerotactic response in a capillary assay. Wild‐type P. putida clustered at the air/liquid interface. In contrast, the aer mutant did not cluster at the interface, but instead formed a diffuse band at a distance from the meniscus. Wild‐type aer , provided in trans , complemented the aer mutant to an aerotactic response that was stronger than wild‐type. The P. putida Aer sequence is similar over its entire length to the aerotaxis (energy taxis) signal transducer protein, Aer, of Escherichia coli . The amino‐terminus is similar to redox‐sensing regulatory proteins, and the carboxy‐terminus contains the highly conserved domain present in chemotactic transducers.