Open AccessClass I integrons containing a dhfrI trimethoprim resistance gene cassette in aquatic Acinetobacter spp.
Author(s) -
Petersen Andreas,
Guardabassi Luca,
Dalsgaard Anders,
Olsen John E
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb08876.x
Subject(s) - integron , gene cassette , biology , microbiology and biotechnology , acinetobacter , gene , genetics , trimethoprim , antibiotic resistance , sulfamethoxazole , antibiotics
The presence of antibiotic resistance gene cassettes in class I integrons was investigated in 24 sulfamethoxazole‐resistant and ‐sensitive Acinetobacter isolates derived from two Danish freshwater trout farms. Integrons were detected in five isolates from one of the fish farms, and their inserts were characterised by DNA sequencing. Each isolate contained a dhfrI gene cassette encoding resistance to trimethoprim and an open reading frame orfC of unknown function identical to the content of an integron previously found in a clinical enterobacterial isolate. Among the five isolates, at least two different strains were differentiated based on phenotypic tests and randomly amplified polymorphic DNA analysis. To our knowledge, this is the first report and characterisation of an integron in environmental bacteria.