Open AccessA practicable and accurate method to differentiate between intra‐ and extracellular water of microbial cells
Author(s) -
Illmer P.,
Erlebach C.,
Schinner F.
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13769.x
Subject(s) - extracellular , intracellular , arthrobacter , extracellular fluid , nutrient , chemistry , microbiology and biotechnology , biology , biochemistry , ecology , enzyme
A thermographimetric method which allows for a quick and accurate estimation of intra‐ and extracellular water of microbial cells is reviewed and improved. Knowledge of these fractions is important for physiological as well as for toxicological investigations. Results of the study indicate that besides the species, nutrient availability and growth conditions affect the intracellular water content. Intra‐ and extracellular water, dry matter, volume and density of a single cell of Arthrobacter sp. are calculated. There are indications that intracellular compartments of eukaryotes could also be investigated with this method.