Open AccessCloning of the rplA gene encoding ribosomal protein L1 from Streptomyces aureofaciens and its transcriptional analysis in the course of differentiation
Author(s) -
Ján Kormanec,
Renáta Nováková,
L'uboš Kl'učár,
Dagmar Homérová,
Beatrica Ševčíková,
Ondrej Sprušanský
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13768.x
Subject(s) - streptomyces aureofaciens , biology , gene , ribosomal protein , microbiology and biotechnology , open reading frame , genetics , streptomyces , rna , peptide sequence , ribosome , bacteria
Using the method for the identification of promoters recognized by the sporulation‐specific σ factor RpoZ, we identified a positive 882‐bp Sau 3AI DNA fragment in Streptomyces aureofaciens . Sequence analysis revealed a complete open reading frame encoding a protein of 242 amino acids with a M r of 25974. The deduced protein product showed high sequence similarity to all ribosomal proteins L1, with maximum similarity to several Streptomyces spp. L1 (97% identity). The 5′‐truncated rplK gene encoding ribosomal protein L11 was located upstream of the rplA gene. However, the putative rpoZ ‐dependent promoter, P ren25 , was located divergently in the coding region of the rplA gene and was inactive in S. aureofaciens . To investigate expression of the rplA gene, Northern blot hybridization was performed using RNA prepared from S. aureofaciens in various developmental stages. These studies suggested that the rplA gene is expressed in all stages of differentiation as a 1400‐nucleotides transcript. The amount of the transcript decreased during differentiation.