Open AccessCloning of two cold shock genes, cspA and cspG , from the deep‐sea psychrophilic bacterium Shewanella violacea strain DSS12
Author(s) -
Fujii Shinsuke,
Nakasone Kaoru,
Horikoshi Koki
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13767.x
Subject(s) - cold shock domain , biology , psychrophile , gene , escherichia coli , cloning (programming) , rna , genetics , microbiology and biotechnology , bacteria , computer science , programming language
We cloned and characterized two cold shock inducible genes from the deep‐sea psychrophilic bacterium Shewanella violacea strain DSS12. The cloned genes, designated cspA and cspG , encode proteins each consisting of 70 amino acid residues which show 62 and 67% sequence identity with Escherichia coli CspA and CspG, respectively. AT‐rich UP elements were found immediately upstream of the promoter region and the cspA and cspG mRNA contained unusually long 5′ untranslated regions like that in the E. coli cspA, cspB, cspG and cspI genes. Following a temperature downshift to 4°C or −1°C, the levels of cspA and cspG mRNA increased and the level of expression of cspG was greater than that of cspA both before and after cold shock. These results suggest that CspA and CspG may function as RNA chaperones, the mRNAs encoded by these two genes may be regulated post‐transcriptionally and they may function as regulators of other cold shock inducible genes like in E. coli .