Open AccessThe broad host range plasmid pLF1311 from Lactobacillus fermentum VKM1311
Author(s) -
Aleshin Vladimir V.,
Semenova Elena V.,
Doroshenko Vera G.,
Jomantas Yurgis V.,
Tarakanov Boris V.,
Livshits Vitaliy A.
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13758.x
Subject(s) - plasmid , lactobacillus fermentum , biology , microbiology and biotechnology , escherichia coli , lactococcus , staphylococcus aureus , lactobacillus , gene , genetics , bacteria , lactococcus lactis , lactic acid , lactobacillus plantarum
The complete nucleotide sequence (2389 bp) of the cryptic plasmid pLF1311 from Lactobacillus fermentum VKM1311 was determined. DNA sequence analysis revealed the putative coding regions for a replicative protein (RepB), its repressor (RepA) and double‐stranded ( dso ) and single‐stranded ( sso ) origins. pLF1311 belongs to the pE194 family of rolling circle‐replicating plasmids. A derivative of pLF1311 that contains the cat gene of plasmid pC194 of Staphylococcus aureus and the oriT of RP4 was constructed and transferred by conjugative mobilization from Escherichia coli to various Gram‐positive bacteria. The stable maintenance of this derivative was shown in some strains of Lactobacillus, Lactococcus, Enterococcus and Bacillus under non‐selective conditions.