Open AccessTranscriptional analysis of the cyclophilin A gene ( cypA ) of Streptomyces chrysomallus
Author(s) -
Berger Rico,
Schauwecker Florian,
Keller Ullrich
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13757.x
Subject(s) - cypa , start codon , biology , cyclophilin , microbiology and biotechnology , transcription (linguistics) , cyclophilin a , shine dalgarno sequence , gene , messenger rna , genetics , linguistics , philosophy
Streptomyces chrysomallus produces a 17.5‐kDa cyclophilin (CypA) as a major cytosolic protein. Northern blotting of total RNA from S. chrysomallus showed that cypA is transcribed as a monocistronic 500‐nt transcript during the vegetative growth phase only. Consistently, Western blot analysis and enzyme activity determinations revealed a high level of CypA which declined drastically when cultures entered postexponential phase. Primer extension experiments revealed that cypA is transcribed as a leaderless transcript with the startpoint of transcription and translation being the same AUG codon. Analysis of −10 and −35 regions revealed an Eσ hrdB ‐specific promoter consensus sequence in accordance with the observed transcription during vegetative growth of cultures. The leaderless cypA mRNA sequence between codons 5 to 12 shows complementarity to an internal antidownstream box of 16S rRNA of Streptomyces lividans . This may indicate a possible interaction of the leaderless cypA transcript with the 16S mRNA in translation initiation.