Open AccessMolecular cloning and analysis of a pleiotropic regulatory gene locus from the nystatin producer Streptomyces noursei ATCC11455
Author(s) -
Sekurova Olga,
Sletta Håvard,
Ellingsen Trond E.,
Valla Svein,
Zotchev Sergey
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13746.x
Subject(s) - regulator gene , biology , aspergillus nidulans , catabolite repression , repressor , gene , actinorhodin , genetics , streptomyces , nystatin , locus (genetics) , regulation of gene expression , streptomyces coelicolor , gene expression , antibiotics , bacteria , mutant
A regulatory gene locus from Streptomyces noursei ATCC14455, the producer of the antifungal antibiotic nystatin, was cloned in Streptomyces lividans based on its ability to activate actinorhodin (Act) production in this host. Deletion and DNA sequencing analyses showed that a small gene, designated ssmA , located downstream of an afsR homologue (a known pleiotropic regulator) was responsible for the Act overproduction in S. lividans . Database searches for the ssmA gene product revealed its limited similarity to the AfsR2 regulatory protein from S. lividans and CREA catabolite repressor from Aspergillus nidulans . To study the effect of ssmA on nystatin production, this gene was either deleted from S. noursei genome, or placed under control of P ermE* promoter and introduced in S. noursei . The properties of the corresponding strains indicate that ssmA is involved in regulation of growth and antibiotic production only in the media with certain carbon sources.