Open AccessIdentification of nodulation promoter ( nod ‐box) regions of Rhizobium galegae
Author(s) -
Suominen Leena,
Paulin Lars,
Saano Aimo,
Saren AriMatti,
Tas Eva,
Lindström Kristina
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13735.x
Subject(s) - biology , cosmid , genetics , nod , sinorhizobium meliloti , gene , tata box , microbiology and biotechnology , promoter , gene expression , mutant
A hybridisation analysis of a genomic clone library of Rhizobium galegae HAMBI 1174 located four Eco RI fragments homologous to the nod ‐box promoter sequence of Sinorhizobium meliloti in two separate gene regions. Two of the five nod ‐boxes detected in the R. galegae genome were carried on a single cosmid clone, pRg30, upstream from the nodABCIJ and nodF genes, whereas the other three nod ‐boxes were carried on a different cosmid clone, pRg10. Hybridisations with various nod gene probes from S. meliloti and Rhizobium leguminosarum species detected a nodD homolog in pRg10. The sequence data obtained from regions adjacent to each nod ‐box in pRg10 confirmed the presence of a second nodD in the R. galegae genome and, in addition, revealed the presence of nodN, nodU, dctA nifH and nifQ ‐like genes in pRg10. Thus, by using a promoter‐specific nod ‐box probe we could identify a new region carrying genes involved in nitrogen fixation and host specificity functions.