Open AccessThe effect of manganese on the production of Phanerochaete flavido‐alba ligninolytic peroxidases in nitrogen limited cultures
Author(s) -
Ben Hamman Omar,
Rubia Teresa,
Martínez José
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13724.x
Subject(s) - phanerochaete , peroxidase , manganese , manganese peroxidase , lignin peroxidase , extracellular , chemistry , lignin , biochemistry , food science , enzyme , organic chemistry
Manganese supplementation of culture medium affected Phanerochaete flavido‐alba FPL 106507 growth, glucose consumption and extracellular protein accumulation. Both the titre and time of detection of lignin peroxidase (LiP) were affected by manganese concentration in the medium, whereas with manganese peroxidase (MnP) only the titre was affected. In high Mn(II) containing cultures highest manganese peroxidase levels and a decrease in extracellular veratryl alcohol accumulation were observed. After FPLC a number of haemprotein peaks showing manganese peroxidase activity were detected in Mn(II) supplemented cultures. On the contrary, only haemprotein peaks of lignin peroxidase were detected in culture medium not supplemented with Mn(II).