Open AccessTransposition of IS 1181 in the genomes of Staphylococcus and Listeria
Author(s) -
Chesneau Olivier,
Lailler Renaud,
Derbise Anne,
El Solh Névine
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13718.x
Subject(s) - biology , transposable element , plasmid , transposase , insertion sequence , replicon , mobile genetic elements , electroporation , microbiology and biotechnology , tetracycline , listeria , transposition (logic) , insertion , inverted repeat , genetics , staphylococcus aureus , gene , listeria monocytogenes , bacteria , genome , mutation , antibiotics , linguistics , philosophy
The recombinant plasmid pIP1713 was constructed to analyse the transpositional activity of the insertion sequence IS 1181 in Staphylococcus aureus RN4220, Staphylococcus carnosus TM300 and Listeria monocytogenes EGD. This 11.3‐kb plasmid contains two genetically different elements: (i) a pE194ts‐derived replicon, the ermC gene of which confers resistance to erythromycin in Gram‐positive bacteria of several species, and (ii) a copy of IS 1181 , cloned from S. aureus BM3121, in which the tetracycline resistance gene, tet (T), has been inserted between the transposase‐encoded gene and the downstream inverted repeat. When introduced by electroporation into the three bacterial hosts, pIP1713 delivered IS 1181 Ω tet (T) to various chromosomal sites. Cointegrate structures between pIP1713 and the host chromosome were occasionally detected. Transposition was associated with 8‐bp repeats at the insertion sites. IS 1181 Ω tet (T) could be used for random mutagenesis in Gram‐positive bacteria.