Open AccessAnalysis of Frankia evolutionary radiation using glnII sequences
Author(s) -
Cournoyer Benoit,
Lavire Céline
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13709.x
Subject(s) - frankia , actinorhizal plant , biology , phylogenetic tree , genome , botany , clone (java method) , gene , symbiosis , phylogenetics , glutamine synthetase , genetics , root nodule , bacteria , glutamine , amino acid
Using a glnII (encoding glutamine synthetase II) PCR selective screening, a Frankia ACN14a gene library clone was isolated. A derived glnII ‐hybridising 2.7‐kb Hin dIII subclone was characterised. Identities of 95% and 93% were observed, respectively, with the corresponding Frankia CpI1 glnI and glnII regions. A variable segment of the glnII region was selected, PCR amplified from various Frankia genomes, sequenced, and used to investigate phylogenetic relationships within the genus. glnII phylogenetic inferences are well‐resolved and allowed us to deduce evolutionary trends among Frankia . Frankia radiation seems to begin with a diversification according to the ability or not to infect actinorhizal plants. The infective strains are divided into two clusters matching plant‐colonising specificities.