Open AccessCharacterisation of Streptococcus thermophilus CNRZ1205 and its cured and re‐lysogenised derivatives
Author(s) -
Stanley Elizabeth,
Fitzgerald Gerald F.,
Sinderen Douwe
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13704.x
Subject(s) - lysogenic cycle , derivative (finance) , strain (injury) , streptococcus thermophilus , gel electrophoresis , temperateness , biology , chemistry , microbiology and biotechnology , stereochemistry , bacteria , bacteriophage , genetics , escherichia coli , gene , anatomy , financial economics , economics , lactobacillus
Streptococcus thermophilus CNRZ1205 is the lysogenic host for the temperate phage ?O1205. A derivative of CNRZ1205 was isolated which was cured of ?O1205 and this strain was used to construct a re‐lysogenised derivative. Pulse field gel electrophoresis and sequencing of the attachment site regions confirmed that excision and re‐integration of the phage was a site‐specific event. Interestingly, cells from the cured, as well as its re‐lysogenised derivative, were found to have a very long chain length.