Open AccessModulation of peroxide stress response by thiol reagents and the role of a redox sensor‐transcription regulator, OxyR in mediating the response in Xanthomonas
Author(s) -
Vattanaviboon Paiboon,
Varaluksit Tanutra,
Mongkolsuk Skorn
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13699.x
Subject(s) - catalase , thiol , activator (genetics) , peroxide , biochemistry , chemistry , reagent , inducer , xanthomonas campestris , bacteria , mutant , oxidative stress , microbiology and biotechnology , biology , gene , organic chemistry , genetics
Pretreatment of Xanthomonas campestris pv. phaseoli with low inducing concentrations of thiol reagents such as N‐ethylmaleimide (NEM) and diamide induced resistance to H 2 O 2 killing. In addition, these compounds were moderate inducers of peroxide detoxification enzymes such as catalase and alkyl hydroperoxide reductase. For both cases, thiol reagent induced responses required a functional redox sensor/transcription activator oxyR and were absent in an oxyR mutant. By contrast, NEM pretreatment enhanced the killing effects of organic peroxide. The observed Xanthomonas physiological responses to thiol reagent pretreatment, and subsequent challenge with peroxide stress, differed from other bacteria.