Open AccessProduction of a recombinant lipase artificially localized on the Bacillus subtilis cell surface
Author(s) -
Tsuchiya Atsushi,
Kobayashi Gota,
Yamamoto Hiroki,
Sekiguchi Junichi
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13686.x
Subject(s) - autolysin , bacillus subtilis , lipase , zymography , biochemistry , extracellular , recombinant dna , triacylglycerol lipase , hydrolase , bacillaceae , microbiology and biotechnology , chemistry , gel electrophoresis , biology , gene , enzyme , bacteria , mutant , genetics
Abstract The lipB gene encoding an extracellular lipase, LipB, from Bacillus subtilis was fused to the 5′‐terminal region of the cwlB gene encoding the cell wall‐binding domain of the major cell wall hydrolase (autolysin). SDS‐polyacrylamide gel electrophoresis and zymography revealed that the fused protein is localized in the B. subtilis cell wall fraction and retains lipase activity.