Open AccessThe effect of some antibiotic‐resistance‐conferring plasmids on the removal of the heat‐aggregated proteins from Escherichia coli cells
Author(s) -
Kȩdzierska Sabina,
Staniszewska Małgorzata,
Potrykus Joanna,
Wȩgrzyn Grzegorz
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13673.x
Subject(s) - escherichia coli , chloramphenicol , chloramphenicol acetyltransferase , kanamycin , plasmid , biology , gene , microbiology and biotechnology , tetracycline , heterologous , biochemistry , reporter gene , chemistry , gene expression , antibiotics
We found that the presence of plasmids expressing tetracycline resistance or chloramphenicol resistance genes, but not those expressing ampicillin resistance or kanamycin resistance genes, in Escherichia coli led to the retardation of the process of removal of the heat‐aggregated proteins (i.e. the S fraction) from the bacterial cells. The presence of chloramphenicol acetyltransferase in the S fraction is demonstrated. Moreover, we observed that the expression of T7 RNA polymerase gene had an influence on S fraction removal. These results suggest that high level production of some heterologous proteins which are accumulated in the cytoplasm, but not proteins exported through the cell membranes, may cause overloading of the S fraction and delay in the removal of heat‐aggregated proteins from bacterial cells.