Open AccessDegradation of Candida albicans Can1 permease expressed in Saccharomyces cerevisiae
Author(s) -
MatějčkováForejtová Alena,
Kinclová Olga,
Sychrová Hana
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13670.x
Subject(s) - permease , saccharomyces cerevisiae , ubiquitin ligase , biochemistry , vacuole , ubiquitin , candida albicans , biology , saccharomyces , mutant , yeast , microbiology and biotechnology , gene , cytoplasm
The Candida albicans amino‐acid Can1 permease expressed in Saccharomyces cerevisiae is degraded in the vacuole after internalisation by endocytosis. The CaCan1 inactivation and degradation is slow and not inducible by ammonium ions or ‘stress’ conditions. Using Saccharomyces cerevisiae mutants defective in ubiquitin‐protein ligase and ubiquitin‐protein hydrolase we have shown that the degradation of heterologous CaCan1 permease is ubiquitin dependent.