Open AccessCo‐expression of 3‐ketoacyl‐ACP reductase and polyhydroxyalkanoate synthase genes induces PHA production in Escherichia coli HB101 strain
Author(s) -
Taguchi Kazunori,
Aoyagi Yoshihiro,
Matsusaki Hiromi,
Fukui Toshiaki,
Doi Yoshiharu
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13660.x
Subject(s) - escherichia coli , polyhydroxyalkanoates , aeromonas caviae , biology , plasmid , bacteria , pseudomonas , pseudomonadaceae , reductase , biochemistry , strain (injury) , pseudomonadales , microbiology and biotechnology , atp synthase , gene , enterobacteriaceae , enzyme , vibrionaceae , genetics , anatomy
The Escherichia coli 3‐ketoacyl‐ACP reductase gene ( fabG Ec ) was cloned using a PCR technique to investigate the metabolic link between fatty acid metabolism and polyhydroxyalkanoate (PHA) production. Three plasmids respectively harboring fabG Ec and the poly‐3‐hydroxyalkanoate synthesis genes phaC Ac and phaC1 Ps from Aeromonas caviae and Pseudomonas sp. 61‐3 respectively were constructed and introduced into E. coli HB101 strain. On a two‐stage cultivation using dodecanoate as the sole carbon source, recombinant E. coli HB101 strains harboring fabG Ec and phaC genes accumulated PHA copolymers (about 8 wt% of dry cell weight) consisting of several ( R )‐3‐hydroxyalkanoate units of C4, C6, C8, and C10. It has been suggested that overexpression of the fabG Ec gene leads to the supply of ( R )‐3‐hydroxyacyl‐CoA for PHA synthesis via fatty acid degradation.