Open AccessAssessment of lux ‐marked Pseudomonas fluorescens for reporting on organic carbon compounds
Author(s) -
Yeomans Catrin V,
Porteous Fiona,
Paterson Eric,
Meharg Andrew A,
Killham Kenneth
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13645.x
Subject(s) - pseudomonas fluorescens , rhizosphere , carbon fibers , population , total organic carbon , carbon source , pseudomonadales , botany , chemistry , environmental chemistry , nutrient , pseudomonas , biology , bacteria , ecology , biochemistry , materials science , genetics , composite number , demography , sociology , composite material
Carbon‐flow from plant roots to the rhizosphere provides a major source of nutrients for the soil microbial population. However, quantification of carbon‐flow is problematic due to its complex composition. This study investigated the potential of lux ‐marked Pseudomonas fluorescens to discriminate between forms of carbon present in the rhizosphere by measuring the light response to a range of carbon compounds. Results indicate that bioluminescence of short‐term carbon‐starved P. fluorescens is dependent upon the source and concentration of carbon. This system, therefore, has the potential to both quantify and qualify organic acids present in rhizodeposits.