Open AccessIdentification of the promoter region of the Xanthomonas campestris pv. citri recA gene responsible for induction by DNA‐damaging agents
Author(s) -
Yang MeiKwei,
Wu PaiI
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13642.x
Subject(s) - subcloning , biology , xanthomonas campestris , plasmid , microbiology and biotechnology , promoter , gene , dna , xanthomonas , pathovar , reporter gene , genetics , gene expression , pseudomonas aeruginosa , pseudomonadaceae , bacteria
The abundance of the RecA protein and of recA transcripts was markedly increased on exposure of Xanthomonas campestris pathovar citri to various DNA‐damaging agents, including mitomycin C. The promoter sequence responsible for mediating the sensitivity of recA expression to DNA damage was investigated by subcloning a 426‐bp restriction fragment of the 5′ untranslated and coding region of the gene into a promoterless vector containing the luxAB genes of Vibrio fischeri. Xanthomonas campestris pv. citri cells transformed with this vector responded to DNA‐damaging agents with a marked increase in luciferase activity. Deletion of nucleotides from the 5′ end of the recA fragment inserted into the reporter plasmid revealed that the 58 bp upstream of the transcription initiation site are sufficient to mediate induction of recA expression by mitomycin C.