Open AccessPhysical and genetic map of the Listeria monocytogenes EGD serotype 1/2a chromosome
Author(s) -
Both Ulrich,
Otten Sonja,
Darbouche Abdelhak,
Domann Eugen,
Chakraborty Trinad
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13632.x
Subject(s) - listeria monocytogenes , biology , listeria , chromosome , genetics , virulence , genome , serotype , pulsed field gel electrophoresis , restriction map , microbiology and biotechnology , gene , bacteria , plasmid , genotype
Listeria monocytogenes is a facultative intracellular pathogen responsible for both invasive and non‐invasive food‐borne illness in animals and humans. In this study, macrorestriction analysis following pulsed‐field gel electrophoresis was used to show that Listeria monocytogenes serovar 1/2a strain EGD has a single chromosome containing eight Not I fragments of 1100, 850, 365, 320, 275, 40, 30 and 20 kb in size and 11 Asc I fragments of 860, 470, 410, 360, 320, 250, 110, 80, 50, 30 and 20 kb. The total genome therefore comprises 3000±50 kb. The creation of a physical and genetic map of the Listeria genome was achieved by generating Not I linking clones and their use in subsequent hybridisation analysis. Using isogenic mutants harbouring additional artificial Not I restriction sites, we were able to precisely map the positions of all currently known virulence genes on the chromosome.