Open AccessChromosome mapping in Cryptosporidium parvum and establishment of a long‐range restriction map for chromosome VI
Author(s) -
Putignani Lorenza,
Sallicandro Pina,
Alano Pietro,
Abrahamsen Mitchell S.,
Crisanti Andrea,
Spano Furio
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13625.x
Subject(s) - biology , karyotype , chromosome , microbiology and biotechnology , southern blot , genetics , restriction map , telomere , gene , plasmid
We used contour‐clamped homogeneous electric field (CHEF) gel electrophoresis and Southern blot hybridization to analyze the molecular karyotype of Cryptosporidium parvum and establish the chromosomal location of 12 single copy genes. In agreement with previous studies, the molecular karyotype of C. parvum was found to consist of partially co‐migrating chromosomes ranging in size from 0.97 to 1.55 Mb and segregating into five distinct electrophoretic bands. Hybridization results allowed the definition of a linkage group comprised of five distinct loci located on chromosome VI. Southern hybridization and restriction analysis of total C. parvum chromosomes or isolated chromosome VI using gene‐specific probes and an oligonucleotide specific for C. parvum telomeres allowed the development of a long‐range restriction map of chromosome VI.