Open AccessIsolation of the murI gene from Brevibacterium lactofermentum ATCC 13869 encoding d ‐glutamate racemase
Author(s) -
Malathi Konganapuram C.,
Wachi Masaaki,
Nagai Kazuo
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13619.x
Subject(s) - isolation (microbiology) , biology , chemistry , microbiology and biotechnology
The murI gene encoding d ‐glutamate racemase plays an important role in the biosynthesis of d ‐glutamic acid, an essential component of cell wall peptidoglycan of almost all eubacteria. A DNA fragment that could rescue the auxotrophy of d ‐glutamic acid in the Escherichia coli murI mutant strain WM335 was isolated from Brevibacterium lactofermentum ATCC 13869 belonging to the coryneform bacteria. DNA sequencing reveals that it encodes a protein of 284 amino acid residues, which shows a high level of homology with d ‐glutamate racemases from several other bacteria.